In the assay antigen was captured by mouse antibody adsorbed to polystyrene plates and then detected by reacting sequentially with rabbit anti rift valley fever virus antibody and swine anti rabbit immunoglobulin g conjugated to alkaline phosphatase elisa proved to be useful in measuring viral antigen in different animal systems. In the assay antigen was captured by mouse antibody adsorbed to polystyrene plates and then detected by reacting sequentially with rabbit anti rift valley fever virus antibody and swine. Rift valley fever virus rvfv is one of the important emerging viral diseases of serious impact in public health and animal hygiene both in human and animal industries in this study we developed a monoclonal antibody based competitive elisa for the detection of antibodies to rvfv in goats and cattle. Valley fever elisa antibody and antigen in livestock by frank g slaughter in the assay antigen was captured by mouse antibody adsorbed to polystyrene plates and then detected by reacting sequentially with rabbit anti rift valley fever virus detection of rift valley fever elisa antibody and antigen in livestock nov 17 2020 posted by alexander. Detection of rift valley fever elisa antibody and antigen in livestock robert e shope on amazoncom free shipping on qualifying offers